zTrap: zebrafish gene trap and enhancer trap database
نویسندگان
چکیده
منابع مشابه
Patterns of gene action in plant development revealed by enhancer trap and gene trap transposable elements.
The crucifer Arabidopsis thaliana has been used widely as a model organism for the study of plant development. We describe here the development of an efficient insertional mutagenesis system in Arabidopsis that permits identification of genes by their patterns of expression during development. Transposable elements of the Ac/Ds system carrying the GUS reporter gene have been designed to act as ...
متن کاملEstablishment of gene-trap and enhancer-trap systems in the moss Physcomitrella patens.
Because of its simple body plan and ease of gene knockout and allele replacement, the moss Physcomitrella patens is often used as a model system for studies in plant physiology and developmental biology. Gene-trap and enhancer-trap systems are useful techniques for cloning genes and enhancers that function in specific tissues or cells. Additionally, these systems are convenient for obtaining mo...
متن کاملContext dependent function of APPb enhancer identified using enhancer trap-containing BACs as transgenes in zebrafish
An enhancer within intron 1 of the amyloid precursor protein gene (APPb) of zebrafish is identified functionally using a novel approach. Bacterial artificial chromosomes (BACs) were retrofitted with enhancer traps, and expressed as transgenes in zebrafish. Expression from both transient assays and stable lines were used for analysis. Although the enhancer was active in specific nonneural cells ...
متن کاملDETECTION OF GENE ENHANCERS BY Drosophila ENHANCER TRAP ANALYSIS
Enhancers: Transcriptional enhancers are DNA sequences that control expression of nearby genes and direct tissue-specific, positive regulation of those genes (Walker & Rapley,1997; also, any contemporary genetics or cell biology textbook). The general mode of action of these sequences is that they associate with specific activator proteins (that are found in only certain tissues); this associat...
متن کاملGAL4 enhancer trap patterns during Drosophila development.
To identify genes expressed in interesting spatial and temporal patterns during development, we mobilized transposons carrying the yeast transcriptional activator, GAL4, to create new insertions throughout the Drosophila genome. At these new sites, neighboring enhancers drive expression of GAL4 in a pattern similar to that of nearby genes (Brand and Perrimon, 1993). Since GAL4 binds to a UAS ta...
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ژورنال
عنوان ژورنال: BMC Developmental Biology
سال: 2010
ISSN: 1471-213X
DOI: 10.1186/1471-213x-10-105